1) Hexokinase: -1 ATP, glucose locked inside cell. Irreversible step 2) PFK1: -1 ATP. Rate-limiting irreversible Step 3) G3P Gehydrogenase (GAPDH): +2 NADH 4) Phosphoglycerate Kinase: +1 ATP 5) Pyruvate Kinase: +1 ATP. Irreversible step"